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KMID : 0377519830080010079
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Chung-Ang Journal of Medicine
1983 Volume.8 No. 1 p.79 ~ p.86
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Studies on the Activity of Isocitrate Dehyrogenase in the Plasma of Normal and Phenylhydrazine-treated Rabbits
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Kim Young-Soo
Lee Dong-Wook
Lee Hi-Sung
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Abstract
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In order to establish the general pattern of synthesis and degradation of plasma isocitrate dehydrogenase(isocitrate: NADP oxidoreductase: EC 1.1.1.42) in normal and reticulocytosic domestic rabbits, sequential measurements of activity of isocitrate dehydrogenase in the plasma of normal and phenylhydrazine treated rabbits were conducted. The activity of isocitrate dehydrogenase was measured by the method of King. The results are summarized as follows ; 1. The reticulocyte count of normal rabbit blood was approximately 2.5%, and the administration of phenylhydrazine resulted in rapid and profound reticulocytosis, i.e., about 63.5% on day 6 and essentially 100% on day 7. 2. The activity of plasma isocitrate dehydrogenase in normal blood was 0.23 unit/ml plasma. 3. In phenylhydrazine treated rabbits, isocitrate dehydiogenase activity was maximal on day 5, about 0.59 unit/ml plasma, which is about 2.3 fold higher than the normal value(K=0.0183; K_d=0.0589; t1/2=17.81 hrs). 4. The rate constants following recovery from phenylhydrazine treatment were K_s=0.0060; K_4 -0.0273 and t1/2=25.38 hrs.
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